Simulation of photochemical hole-burning experiments on photosynthetic reaction centers

Photochemical Hole-burning in Photosynthetic Reaction Centers

The change in absorbance (hole spectrum) of the primary electron donor (P870) in bacterial photosynthetic reaction centers has been studied at 1.5-2.1 K following narrow-band excitation at several wavelengths within the P870 absorption band. The hole width is very large, suggesting a homogeneous linewidth on the order of 200-300 cm-‘. Possible interpretations of this highly unusual result, incl...

Fluorescence and photochemical quenching in photosynthetic reaction centers.

Photosynthetic bacteria contain bacteriochlorophyll (BChl) that absorbs light and delivers the resulting singlet excitation energy to photochemical reaction centers.' The reaction centers, which have been isolated in a form free of lightharvesting BChl,'-3 contain a photochemically specialized BChl which in Rhodopseudomonas spheroides is called P870 after its absorption maximum near 870 nm. Exc...

Photosynthetic reaction centers.

The capture of solar radiation and the conversion of its free energy into chemical energy involves a sequence of reactions that occur within a physical structure called the photosynthetic RC. Following the initial capture of a photon by antenna pigments, the photon is transferred to the RC pigments, where it gives rise to a separation and stabilization of charge across the photosynthetic membra...

Reaction centers of photosynthetic bacteria.

Photochemical Hole-Burning Spectroscopy of Bovine Rhodopsin and Bacterlorhodopsln

Photochemical hole burning has been observed at 1.5 K in bacteriorhodopsin (bR) and bovine rhodopsin at wavelengths spanning their lowest energy electronic absorption bands. Hole widths were -2500 cm-' full width at half-maximum (fwhm) in bacteriorhodopsin and -2400 cm-' fwhm in rhodopsin. The positions of the hole minima were insensitive to bum wavelength, indicating that minimal site selectio...